Transcriptomic and proteomic analysis of mouse radiation–induced acute myeloid leukaemia (AML)

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Transcriptomic and proteomic analysis of mouse radiation–induced acute myeloid leukaemia (AML). / Michaux, Arlette; Benotmane, Rafi; Badie, Christophe; Blachowicz, Agnieszka; Barjaktarovic, Zarko; Finnon, Rosemary; Sarioglu, Hakan; Brown, Natalie; Manning, Grainne; Tapio, Soile; Polanska, Joanna; Bouffler, Simon D.

In: Oncotarget - Impact Journals, Vol. 7, No. 26, 26.05.2016, p. 40461-40480.

Research output: Contribution to journalArticle

Harvard

Michaux, A, Benotmane, R, Badie, C, Blachowicz, A, Barjaktarovic, Z, Finnon, R, Sarioglu, H, Brown, N, Manning, G, Tapio, S, Polanska, J & Bouffler, SD 2016, 'Transcriptomic and proteomic analysis of mouse radiation–induced acute myeloid leukaemia (AML)', Oncotarget - Impact Journals, vol. 7, no. 26, pp. 40461-40480. https://doi.org/10.18632/oncotarget.9626

APA

Michaux, A., Benotmane, R., Badie, C., Blachowicz, A., Barjaktarovic, Z., Finnon, R., ... Bouffler, S. D. (2016). Transcriptomic and proteomic analysis of mouse radiation–induced acute myeloid leukaemia (AML). Oncotarget - Impact Journals, 7(26), 40461-40480. https://doi.org/10.18632/oncotarget.9626

Vancouver

Michaux A, Benotmane R, Badie C, Blachowicz A, Barjaktarovic Z, Finnon R et al. Transcriptomic and proteomic analysis of mouse radiation–induced acute myeloid leukaemia (AML). Oncotarget - Impact Journals. 2016 May 26;7(26):40461-40480. https://doi.org/10.18632/oncotarget.9626

Author

Michaux, Arlette ; Benotmane, Rafi ; Badie, Christophe ; Blachowicz, Agnieszka ; Barjaktarovic, Zarko ; Finnon, Rosemary ; Sarioglu, Hakan ; Brown, Natalie ; Manning, Grainne ; Tapio, Soile ; Polanska, Joanna ; Bouffler, Simon D. / Transcriptomic and proteomic analysis of mouse radiation–induced acute myeloid leukaemia (AML). In: Oncotarget - Impact Journals. 2016 ; Vol. 7, No. 26. pp. 40461-40480.

Bibtex - Download

@article{485fad1babc746b5a0ed287d4a30d3e5,
title = "Transcriptomic and proteomic analysis of mouse radiation–induced acute myeloid leukaemia (AML)",
abstract = "A combined transcriptome and proteome analysis of mouse radiation-induced AMLs using two primary AMLs, cell lines from these primaries, another cell line and its in vivo passage is reported. Compared to haematopoietic progenitor and stem cells (HPSC), over 5000 transcriptome alterations were identified, 2600 present in all materials. 55 and 3 alterations were detected in the proteomes of the cell lines and primary/in vivo passage material respectively, with one common to all materials. In cell lines, approximately 50{\%} of the transcriptome changes are related to adaptation to cell culture, and in the proteome this proportion was higher. An AML ‘signature’ of 17 genes/proteins commonly deregulated in primary AMLs and cell lines compared to HPSCs was identified and validated using human AML transcriptome data. This also distinguishes primary AMLs from cell lines and includes proteins such as Coronin 1, pontin/RUVBL1 and Myeloperoxidase commonly implicated in human AML. C-Myc was identified as having a key role in radiation leukaemogenesis. These data identify novel candidates relevant to mouse radiation AML pathogenesis, and confirm that pathways of leukaemogenesis in the mouse and human share substantial commonality.",
keywords = "ionising radiation, acute myeloid leukaemia, mouse, gene expression, protein expression",
author = "Arlette Michaux and Rafi Benotmane and Christophe Badie and Agnieszka Blachowicz and Zarko Barjaktarovic and Rosemary Finnon and Hakan Sarioglu and Natalie Brown and Grainne Manning and Soile Tapio and Joanna Polanska and Bouffler, {Simon D.}",
note = "Score=10",
year = "2016",
month = "5",
day = "26",
doi = "10.18632/oncotarget.9626",
language = "English",
volume = "7",
pages = "40461--40480",
journal = "Oncotarget - Impact Journals",
issn = "1949-2553",
publisher = "Impact Journals",
number = "26",

}

RIS - Download

TY - JOUR

T1 - Transcriptomic and proteomic analysis of mouse radiation–induced acute myeloid leukaemia (AML)

AU - Michaux, Arlette

AU - Benotmane, Rafi

AU - Badie, Christophe

AU - Blachowicz, Agnieszka

AU - Barjaktarovic, Zarko

AU - Finnon, Rosemary

AU - Sarioglu, Hakan

AU - Brown, Natalie

AU - Manning, Grainne

AU - Tapio, Soile

AU - Polanska, Joanna

AU - Bouffler, Simon D.

N1 - Score=10

PY - 2016/5/26

Y1 - 2016/5/26

N2 - A combined transcriptome and proteome analysis of mouse radiation-induced AMLs using two primary AMLs, cell lines from these primaries, another cell line and its in vivo passage is reported. Compared to haematopoietic progenitor and stem cells (HPSC), over 5000 transcriptome alterations were identified, 2600 present in all materials. 55 and 3 alterations were detected in the proteomes of the cell lines and primary/in vivo passage material respectively, with one common to all materials. In cell lines, approximately 50% of the transcriptome changes are related to adaptation to cell culture, and in the proteome this proportion was higher. An AML ‘signature’ of 17 genes/proteins commonly deregulated in primary AMLs and cell lines compared to HPSCs was identified and validated using human AML transcriptome data. This also distinguishes primary AMLs from cell lines and includes proteins such as Coronin 1, pontin/RUVBL1 and Myeloperoxidase commonly implicated in human AML. C-Myc was identified as having a key role in radiation leukaemogenesis. These data identify novel candidates relevant to mouse radiation AML pathogenesis, and confirm that pathways of leukaemogenesis in the mouse and human share substantial commonality.

AB - A combined transcriptome and proteome analysis of mouse radiation-induced AMLs using two primary AMLs, cell lines from these primaries, another cell line and its in vivo passage is reported. Compared to haematopoietic progenitor and stem cells (HPSC), over 5000 transcriptome alterations were identified, 2600 present in all materials. 55 and 3 alterations were detected in the proteomes of the cell lines and primary/in vivo passage material respectively, with one common to all materials. In cell lines, approximately 50% of the transcriptome changes are related to adaptation to cell culture, and in the proteome this proportion was higher. An AML ‘signature’ of 17 genes/proteins commonly deregulated in primary AMLs and cell lines compared to HPSCs was identified and validated using human AML transcriptome data. This also distinguishes primary AMLs from cell lines and includes proteins such as Coronin 1, pontin/RUVBL1 and Myeloperoxidase commonly implicated in human AML. C-Myc was identified as having a key role in radiation leukaemogenesis. These data identify novel candidates relevant to mouse radiation AML pathogenesis, and confirm that pathways of leukaemogenesis in the mouse and human share substantial commonality.

KW - ionising radiation

KW - acute myeloid leukaemia

KW - mouse

KW - gene expression

KW - protein expression

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/20890069

U2 - 10.18632/oncotarget.9626

DO - 10.18632/oncotarget.9626

M3 - Article

VL - 7

SP - 40461

EP - 40480

JO - Oncotarget - Impact Journals

JF - Oncotarget - Impact Journals

SN - 1949-2553

IS - 26

ER -

ID: 1915786