Transcriptomic profiling suggests a role for IGFBP5 in premature senescence of endothelial cells after chronic low dose rate irradiation

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Transcriptomic profiling suggests a role for IGFBP5 in premature senescence of endothelial cells after chronic low dose rate irradiation. / Rombouts, Charlotte; Aerts, An; Quintens, Roel; Baselet, Bjorn; El Saghire, Houssein; Harms-Ringdahl, Mats; Haghdoost, Siamak; Janssen, Ann; Michaux, Arlette; Yentrapalli, Ramesh; Benotmane, Rafi; Van Oostveldt, Patrick; Baatout, Sarah.

In: International Journal of Radiation Biology, Vol. 90, No. 7, 07.2014, p. 560-574.

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@article{a68674b049b447c4823bc947258eabab,
title = "Transcriptomic profiling suggests a role for IGFBP5 in premature senescence of endothelial cells after chronic low dose rate irradiation",
abstract = "Ionizing radiation has been recognized to increase the risk of cardiovascular diseases. However, there is no consensus concerning the dose-risk relationship for low radiation doses and a mechanistic understanding of low dose effects is needed. Material and methods: Previously, human umbilical vein endothelial cells were exposed to chronic low dose rate radiation (1.4 and 4.1mGy/h) during one, three and six weeks which resulted in premature senescence in cells exposed to 4.1mGy/h. To gain more insight into the underlying signaling pathways, we analyzed gene expression changes in these cells using microarray technology. The obtained data were analyzed in a dual approach, combining single gene expression analysis and Gene Set Enrichment Analysis. Results: An early stress response was observed after one week of exposure to 4.1mGy/h which was replaced by a more inflammation-related expression profile after three weeks and onwards. This early stress response may trigger the radiation-induced premature senescence previously observed in HUVEC irradiated with 4.1mGy/h. A dedicated analysis pointed to the involvement of insulin-like growth factor binding protein 5 (IGFBP5) signaling in radiation-induced premature senescence. Conclusion: Our findings motivate further research on the shape of the dose-response and the dose rate effect for radiation- induced vascular senescence.",
keywords = "Gene expression, chronic low dose rate ionizing radiation, endothelial cells, senescence",
author = "Charlotte Rombouts and An Aerts and Roel Quintens and Bjorn Baselet and {El Saghire}, Houssein and Mats Harms-Ringdahl and Siamak Haghdoost and Ann Janssen and Arlette Michaux and Ramesh Yentrapalli and Rafi Benotmane and {Van Oostveldt}, Patrick and Sarah Baatout",
note = "Score = 10",
year = "2014",
month = jul,
doi = "10.3109/09553002.2014.905724",
language = "English",
volume = "90",
pages = "560--574",
journal = "International Journal of Radiation Biology",
issn = "0955-3002",
publisher = "Taylor & Francis (CRC)",
number = "7",

}

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TY - JOUR

T1 - Transcriptomic profiling suggests a role for IGFBP5 in premature senescence of endothelial cells after chronic low dose rate irradiation

AU - Rombouts, Charlotte

AU - Aerts, An

AU - Quintens, Roel

AU - Baselet, Bjorn

AU - El Saghire, Houssein

AU - Harms-Ringdahl, Mats

AU - Haghdoost, Siamak

AU - Janssen, Ann

AU - Michaux, Arlette

AU - Yentrapalli, Ramesh

AU - Benotmane, Rafi

AU - Van Oostveldt, Patrick

AU - Baatout, Sarah

N1 - Score = 10

PY - 2014/7

Y1 - 2014/7

N2 - Ionizing radiation has been recognized to increase the risk of cardiovascular diseases. However, there is no consensus concerning the dose-risk relationship for low radiation doses and a mechanistic understanding of low dose effects is needed. Material and methods: Previously, human umbilical vein endothelial cells were exposed to chronic low dose rate radiation (1.4 and 4.1mGy/h) during one, three and six weeks which resulted in premature senescence in cells exposed to 4.1mGy/h. To gain more insight into the underlying signaling pathways, we analyzed gene expression changes in these cells using microarray technology. The obtained data were analyzed in a dual approach, combining single gene expression analysis and Gene Set Enrichment Analysis. Results: An early stress response was observed after one week of exposure to 4.1mGy/h which was replaced by a more inflammation-related expression profile after three weeks and onwards. This early stress response may trigger the radiation-induced premature senescence previously observed in HUVEC irradiated with 4.1mGy/h. A dedicated analysis pointed to the involvement of insulin-like growth factor binding protein 5 (IGFBP5) signaling in radiation-induced premature senescence. Conclusion: Our findings motivate further research on the shape of the dose-response and the dose rate effect for radiation- induced vascular senescence.

AB - Ionizing radiation has been recognized to increase the risk of cardiovascular diseases. However, there is no consensus concerning the dose-risk relationship for low radiation doses and a mechanistic understanding of low dose effects is needed. Material and methods: Previously, human umbilical vein endothelial cells were exposed to chronic low dose rate radiation (1.4 and 4.1mGy/h) during one, three and six weeks which resulted in premature senescence in cells exposed to 4.1mGy/h. To gain more insight into the underlying signaling pathways, we analyzed gene expression changes in these cells using microarray technology. The obtained data were analyzed in a dual approach, combining single gene expression analysis and Gene Set Enrichment Analysis. Results: An early stress response was observed after one week of exposure to 4.1mGy/h which was replaced by a more inflammation-related expression profile after three weeks and onwards. This early stress response may trigger the radiation-induced premature senescence previously observed in HUVEC irradiated with 4.1mGy/h. A dedicated analysis pointed to the involvement of insulin-like growth factor binding protein 5 (IGFBP5) signaling in radiation-induced premature senescence. Conclusion: Our findings motivate further research on the shape of the dose-response and the dose rate effect for radiation- induced vascular senescence.

KW - Gene expression

KW - chronic low dose rate ionizing radiation

KW - endothelial cells

KW - senescence

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_135884

UR - http://knowledgecentre.sckcen.be/so2/bibref/11633

U2 - 10.3109/09553002.2014.905724

DO - 10.3109/09553002.2014.905724

M3 - Article

VL - 90

SP - 560

EP - 574

JO - International Journal of Radiation Biology

JF - International Journal of Radiation Biology

SN - 0955-3002

IS - 7

ER -

ID: 275011