Unraveling uranium induced oxidative stress related responses in Arabidopsis thaliana seedlings. Part I: responses in the roots

Research output: Contribution to journalArticlepeer-review

Standard

Unraveling uranium induced oxidative stress related responses in Arabidopsis thaliana seedlings. Part I: responses in the roots. / Vanhoudt, Nathalie; Vandenhove, Hildegarde; Horemans, Nele; Remans, Tony; Opdenakker, Kelly; Smeets, Karen; Martinez Bello, Daniel; Wannijn, Jean; Van Hees, May; Vangronsveld, Jaco; Cuypers, Ann.

In: Journal of environmental radioactivity, Vol. 102, No. 6, 03.2011, p. 630-637.

Research output: Contribution to journalArticlepeer-review

Harvard

Vanhoudt, N, Vandenhove, H, Horemans, N, Remans, T, Opdenakker, K, Smeets, K, Martinez Bello, D, Wannijn, J, Van Hees, M, Vangronsveld, J & Cuypers, A 2011, 'Unraveling uranium induced oxidative stress related responses in Arabidopsis thaliana seedlings. Part I: responses in the roots', Journal of environmental radioactivity, vol. 102, no. 6, pp. 630-637. https://doi.org/10.1016/j.jenvrad.2011.03.015

APA

Vanhoudt, N., Vandenhove, H., Horemans, N., Remans, T., Opdenakker, K., Smeets, K., Martinez Bello, D., Wannijn, J., Van Hees, M., Vangronsveld, J., & Cuypers, A. (2011). Unraveling uranium induced oxidative stress related responses in Arabidopsis thaliana seedlings. Part I: responses in the roots. Journal of environmental radioactivity, 102(6), 630-637. https://doi.org/10.1016/j.jenvrad.2011.03.015

Author

Vanhoudt, Nathalie ; Vandenhove, Hildegarde ; Horemans, Nele ; Remans, Tony ; Opdenakker, Kelly ; Smeets, Karen ; Martinez Bello, Daniel ; Wannijn, Jean ; Van Hees, May ; Vangronsveld, Jaco ; Cuypers, Ann. / Unraveling uranium induced oxidative stress related responses in Arabidopsis thaliana seedlings. Part I: responses in the roots. In: Journal of environmental radioactivity. 2011 ; Vol. 102, No. 6. pp. 630-637.

Bibtex - Download

@article{c25fc68068984b789904f589eceb2592,
title = "Unraveling uranium induced oxidative stress related responses in Arabidopsis thaliana seedlings. Part I: responses in the roots",
abstract = "This study aimed to investigate oxidative stress related responses in Arabidopsis thaliana exposed to uranium concentrations ranging from 0.1 to 100 µM for 1, 3 and 7 days. Results are reported separately for roots and leaves in two papers. Results of Part I indicate that oxidative stress related responses in the roots were only triggered following exposure to the highest uranium concentration of 100 µM. A fast oxidative burst was suggested based on the observed enhancement of lipoxygenase (LOX1) and respiratory burst oxydase homolog (RBOHD) transcript levels already after 1 day. The first line of defense was attributed to superoxide dismutase (SOD), also triggered from the first day. The enhanced SOD-capacity observed at protein level corresponded with an enhanced expression of iron SOD (FSD1) located in the plastids. For the detoxification of H2O2, an early increase in catalase (CAT1) transcript levels was observed while peroxidase capacities were enhanced at the later stage of 3 days. Although the ascorbate peroxidase capacity and gene expression (APX1) increased, the ascorbate/dehydroascorbate redox balance was completely disrupted and shifted towards the oxidized form. This disrupted balance could not be inverted by the glutathione part of the cycle although the glutathione redox balance could be maintained.",
keywords = "Antioxidative defense system, Arabidopsis thaliana, gene expression, oxidative stress, uranium toxicity",
author = "Nathalie Vanhoudt and Hildegarde Vandenhove and Nele Horemans and Tony Remans and Kelly Opdenakker and Karen Smeets and {Martinez Bello}, Daniel and Jean Wannijn and {Van Hees}, May and Jaco Vangronsveld and Ann Cuypers",
note = "Score = 10",
year = "2011",
month = mar,
doi = "10.1016/j.jenvrad.2011.03.015",
language = "English",
volume = "102",
pages = "630--637",
journal = "Journal of environmental radioactivity",
issn = "0265-931X",
publisher = "Elsevier",
number = "6",

}

RIS - Download

TY - JOUR

T1 - Unraveling uranium induced oxidative stress related responses in Arabidopsis thaliana seedlings. Part I: responses in the roots

AU - Vanhoudt, Nathalie

AU - Vandenhove, Hildegarde

AU - Horemans, Nele

AU - Remans, Tony

AU - Opdenakker, Kelly

AU - Smeets, Karen

AU - Martinez Bello, Daniel

AU - Wannijn, Jean

AU - Van Hees, May

AU - Vangronsveld, Jaco

AU - Cuypers, Ann

N1 - Score = 10

PY - 2011/3

Y1 - 2011/3

N2 - This study aimed to investigate oxidative stress related responses in Arabidopsis thaliana exposed to uranium concentrations ranging from 0.1 to 100 µM for 1, 3 and 7 days. Results are reported separately for roots and leaves in two papers. Results of Part I indicate that oxidative stress related responses in the roots were only triggered following exposure to the highest uranium concentration of 100 µM. A fast oxidative burst was suggested based on the observed enhancement of lipoxygenase (LOX1) and respiratory burst oxydase homolog (RBOHD) transcript levels already after 1 day. The first line of defense was attributed to superoxide dismutase (SOD), also triggered from the first day. The enhanced SOD-capacity observed at protein level corresponded with an enhanced expression of iron SOD (FSD1) located in the plastids. For the detoxification of H2O2, an early increase in catalase (CAT1) transcript levels was observed while peroxidase capacities were enhanced at the later stage of 3 days. Although the ascorbate peroxidase capacity and gene expression (APX1) increased, the ascorbate/dehydroascorbate redox balance was completely disrupted and shifted towards the oxidized form. This disrupted balance could not be inverted by the glutathione part of the cycle although the glutathione redox balance could be maintained.

AB - This study aimed to investigate oxidative stress related responses in Arabidopsis thaliana exposed to uranium concentrations ranging from 0.1 to 100 µM for 1, 3 and 7 days. Results are reported separately for roots and leaves in two papers. Results of Part I indicate that oxidative stress related responses in the roots were only triggered following exposure to the highest uranium concentration of 100 µM. A fast oxidative burst was suggested based on the observed enhancement of lipoxygenase (LOX1) and respiratory burst oxydase homolog (RBOHD) transcript levels already after 1 day. The first line of defense was attributed to superoxide dismutase (SOD), also triggered from the first day. The enhanced SOD-capacity observed at protein level corresponded with an enhanced expression of iron SOD (FSD1) located in the plastids. For the detoxification of H2O2, an early increase in catalase (CAT1) transcript levels was observed while peroxidase capacities were enhanced at the later stage of 3 days. Although the ascorbate peroxidase capacity and gene expression (APX1) increased, the ascorbate/dehydroascorbate redox balance was completely disrupted and shifted towards the oxidized form. This disrupted balance could not be inverted by the glutathione part of the cycle although the glutathione redox balance could be maintained.

KW - Antioxidative defense system

KW - Arabidopsis thaliana

KW - gene expression

KW - oxidative stress

KW - uranium toxicity

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/ezp_113624

UR - http://knowledgecentre.sckcen.be/so2/bibref/8023

U2 - 10.1016/j.jenvrad.2011.03.015

DO - 10.1016/j.jenvrad.2011.03.015

M3 - Article

VL - 102

SP - 630

EP - 637

JO - Journal of environmental radioactivity

JF - Journal of environmental radioactivity

SN - 0265-931X

IS - 6

ER -

ID: 82284