Uranium exposure induces nitric oxide and hydrogen peroxide generation in arabidopsis thaliana

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Uranium exposure induces nitric oxide and hydrogen peroxide generation in arabidopsis thaliana. / Tewari, Rajesh; Horemans, Nele; Nauts, Robin; Wannijn, Jean; Van Hees, May; Vandenhove, Hildegarde.

In: Environmental and Experimental Botany, Vol. 120, 15.08.2015, p. 55-64.

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@article{870087945fc445b4890474f720d0b371,
title = "Uranium exposure induces nitric oxide and hydrogen peroxide generation in arabidopsis thaliana",
abstract = "Uranium (U) is a nonessential and toxic heavy metal and radionuclide. Nitric oxide (NO) and hydrogen peroxide (H2O2) are intimately involved in the growth, development and metabolic function of plant cells. In addition to possible toxic activities, a role for these signalling molecules in the regulation of plant responses to toxic metals has been proposed. Here, we investigated the synthesis of NO and H2O2 in Arabidopsis thaliana seedlings exposed to 25 µM U. We demonstrated that U induces NO and H2O2 production in the roots and leaves. Addition of the NO releasing compound, sodium nitroprusside (SNP) as well as the NO synthase inhibitor (L-NAME) to the plants, resulted in a decrease of the U-induced NO production and both alleviated U toxicity as indicated by plant growth (leaf area and biomass). Treatment with L-NAME further resulted in the disappearance of the U-induced H2O2 in the shoots of the plants. It appears that generation of NO and H2O2 is intimately linked in the toxicity response of U-exposed A. thaliana plants.",
keywords = "Arabidopsis thaliana, hydrogen peroxide, lipid peroxidation, nitric oxide, uranium toxicity",
author = "Rajesh Tewari and Nele Horemans and Robin Nauts and Jean Wannijn and {Van Hees}, May and Hildegarde Vandenhove",
note = "Score=10",
year = "2015",
month = aug,
day = "15",
doi = "10.1016/j.envexpbot.2015.08.004",
language = "English",
volume = "120",
pages = "55--64",
journal = "Environmental and Experimental Botany",
issn = "0098-8472",
publisher = "Elsevier",

}

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TY - JOUR

T1 - Uranium exposure induces nitric oxide and hydrogen peroxide generation in arabidopsis thaliana

AU - Tewari, Rajesh

AU - Horemans, Nele

AU - Nauts, Robin

AU - Wannijn, Jean

AU - Van Hees, May

AU - Vandenhove, Hildegarde

N1 - Score=10

PY - 2015/8/15

Y1 - 2015/8/15

N2 - Uranium (U) is a nonessential and toxic heavy metal and radionuclide. Nitric oxide (NO) and hydrogen peroxide (H2O2) are intimately involved in the growth, development and metabolic function of plant cells. In addition to possible toxic activities, a role for these signalling molecules in the regulation of plant responses to toxic metals has been proposed. Here, we investigated the synthesis of NO and H2O2 in Arabidopsis thaliana seedlings exposed to 25 µM U. We demonstrated that U induces NO and H2O2 production in the roots and leaves. Addition of the NO releasing compound, sodium nitroprusside (SNP) as well as the NO synthase inhibitor (L-NAME) to the plants, resulted in a decrease of the U-induced NO production and both alleviated U toxicity as indicated by plant growth (leaf area and biomass). Treatment with L-NAME further resulted in the disappearance of the U-induced H2O2 in the shoots of the plants. It appears that generation of NO and H2O2 is intimately linked in the toxicity response of U-exposed A. thaliana plants.

AB - Uranium (U) is a nonessential and toxic heavy metal and radionuclide. Nitric oxide (NO) and hydrogen peroxide (H2O2) are intimately involved in the growth, development and metabolic function of plant cells. In addition to possible toxic activities, a role for these signalling molecules in the regulation of plant responses to toxic metals has been proposed. Here, we investigated the synthesis of NO and H2O2 in Arabidopsis thaliana seedlings exposed to 25 µM U. We demonstrated that U induces NO and H2O2 production in the roots and leaves. Addition of the NO releasing compound, sodium nitroprusside (SNP) as well as the NO synthase inhibitor (L-NAME) to the plants, resulted in a decrease of the U-induced NO production and both alleviated U toxicity as indicated by plant growth (leaf area and biomass). Treatment with L-NAME further resulted in the disappearance of the U-induced H2O2 in the shoots of the plants. It appears that generation of NO and H2O2 is intimately linked in the toxicity response of U-exposed A. thaliana plants.

KW - Arabidopsis thaliana

KW - hydrogen peroxide

KW - lipid peroxidation

KW - nitric oxide

KW - uranium toxicity

UR - http://ecm.sckcen.be/OTCS/llisapi.dll/open/12030852

U2 - 10.1016/j.envexpbot.2015.08.004

DO - 10.1016/j.envexpbot.2015.08.004

M3 - Article

VL - 120

SP - 55

EP - 64

JO - Environmental and Experimental Botany

JF - Environmental and Experimental Botany

SN - 0098-8472

ER -

ID: 875211